UdderCheckTM measures Lactate Dehydrogenase (LDH), an
enzyme
present in milk when cells are damaged during
an udder infection. LDH is correlated to SCC,
but is
not as easily affected by other conditions such as
stress, nutrition, parity, stage of lactation, etc.
LDH levels often rise earlier than somatic cell counts,
making it an excellent marker for early detection
of
udder infections. Several milking systems have recently
incorporated the measurement of LDH as
an early
indicator of mastitis and to monitor udder health.
The reagent pad on the test strip contains the
immobilized substrate, L-lactate. Through a series of
coupled enzymatic
reactions, this substrate is
oxidized by lactate dehydrogenase (LDH) in the milk,
while simultaneously, the indicator
nitrotetrazolium
blue is reduced to a purple formazan. The end color
intensity of the formazan is proportional to the
concentration of lactate dehydrogenase in milk.
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